Nanosensors in theranostics of diseases

Big nanodiamonds (~100nm) inside colon cancer cells (CT26)
Big nanodiamonds (~100nm) inside colon cancer cells (CT26)

ADVANTAGES

  • small size (5-100 nm) – penetrationthrough the cell membrane
  • easy surface functionalisation
  • intrinsic fluorescence; charged nitrogen vacancy centers (NV);Ex 561/ Em 575 (NV0)and 638 (NV)
  • direct detection of Raman signal with a spectrometer and/or confocal microscopy
  • photostability (no „bleaching“)

 

Usage of ND for phototerapy, thermoterapy, radiotherapy

  • DELIVERY VEHICLES for targeted therapy
  • NON-INVASIVE DISEASE MONITORING and bio-imaging

 

Usage of ND in tumor diagnosis and therapy

  • breast cancer – targeting molecule to Her2/neu (Herceptin)
  • fND – Her2/neu antigene complementary peptid

 

Accumulation of nanoparticles (red signal) in the cytoplasm of breast cancer cells (MDA-MB-231)
Accumulation of nanoparticles (red signal) in the cytoplasm of breast cancer cells (MDA-MB-231)

 

Plain ND; human PBMC - adherent population, 40x ,4x zoom, Hoechst
Plain ND; human PBMC – adherent population, 40x ,4x zoom, Hoechst

 

NCD layer O-terminated; IC-21, 20x, DAPI, ND layers, CVD; O x H terminated
NCD layer O-terminated; IC-21, 20x, DAPI, ND layers, CVD; O x H terminated

 

Expression of eGFP introduced into macrophage lineage IC-21 using FND. Red signal: FND, green signal: GFP expression, yellow signal: FND and colocalization of GFP expression.
Expression of eGFP introduced into macrophage lineage IC-21 using FND. Red signal: FND, green signal: GFP expression, yellow signal: FND and colocalization of GFP expression.
Monitoring the entry of microRNA-21 bound to the nanoparticle (complex - yellow signal nanoparticles - red signal) to colon cancer cells (HT-29).
Monitoring the entry of microRNA-21 bound to the nanoparticle (complex – yellow signal nanoparticles – red signal) to colon cancer cells (HT-29 and CT26).